Calculations For Dual Luciferase Assays

H19 Dye for DNA Relaxation and Supercoiling Assays
DSA1000D 1,000 assays
EUR 488.62
Description: This product includes 170 ul of 1500 x H19 dye for high throughput DNA relaxation or DNA supercoiling assays of topoisomerases.

Human IgG antibody Laboratories manufactures the calculations for dual luciferase assays reagents distributed by Genprice. The Calculations For Dual Luciferase Assays reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact luciferase assay. Other Calculations products are available in stock. Specificity: Calculations Category: For Group: Dual Luciferase

Human thymidylate kinase (TMK) - for 500 assays
500 assays
EUR 960.92
Description: This product includes 20 ul of 50 uM human thymidylate kinase.

Human DNA topoisomerase I - for 10,000 assays
10,000 assays
EUR 867.26
Description: This product includes 20 ul of 2,000 U/ul of purified human Topoisomerase I form sf9 cells and 4 ml of 10 x assay buffer.

Human mitochondrial RNA polymerase - for 100 assays
100 assays
EUR 464.61
Description: This product includes 33 ul of 7.5 uM Human Mitochondrial RNA Polymerase

E. coli BirA - for 100 assays
100 assays
EUR 337.25
Description: This product includes 35 ul of 5 uM E. coli BirA.

E. coli IspD - for 100 assays
100 assays
EUR 337.25
Description: This product includes 35 ul of 200 nM E. coli IspD.

P. aeruginosa IspD - for 100 assays
100 assays
EUR 337.25
Description: This product includes 35 ul of 200 nM P. aeruginosa IspD.

E. coli GlmU - for 100 assays
100 assays
EUR 337.25
Description: This product includes 33 ul of 500 nM E. coli GlmU.

Dual Luciferase information

Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 Assays
30081-2 1000AS
EUR 1066
Description: BSA from bovine serum (cow; Bos taurus)

Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 Assays
30081-2-1 KT
EUR 1066

Firefly & Renilla Luciferase Single Tube Assay Kit, 150 Assays
30081-1 150AS
EUR 220
Description: BSA from bovine serum (cow; Bos taurus)

Firefly & Renilla Luciferase Single Tube Assay Kit, 150 Assays
30081-1-1 KT
EUR 220

Firefly & Renilla Luciferase Single Tube Assay Kit, 50 Assays
30081-T 50AS
EUR 121
Description: BSA from bovine serum (cow; Bos taurus)

Firefly & Renilla Luciferase Single Tube Assay Kit, 50 Assays
30081-T-1 KT
EUR 121

Lysis buffer for luciferase assay 5X
IBS-BL002 50 mL
EUR 49

Blank (Control) Dual Luciferase Stable Cell Line
m033 106 cells/ml, 1.0 ml
EUR 1704

ADAR1 Dual Luciferase Reporter HEK293 Cell Line
78547 2 vials
EUR 19950
Description: The ADAR1 Luciferase Reporter HEK293 cell line is designed to monitor RNA editing by Adenosine deaminase acting on RNA (ADAR1). This cell line stably expresses ADAR1 under the control of a CMV promoter and a separate ADAR editing reporter construct expressed under the control of another CMV promoter. The reporter contains the gene encoding firefly luciferase, which is constitutively expressed in the cells, upstream of the gene encoding the GluA2 ADAR substrate followed by the Renilla luciferase gene. The sequence corresponding to GluA2 has been modified to contain an amber stop codon (UAG). When edited by ADAR, this stop codon (UAG) will be changed to UIG (A to I edit), which is read as tryptophan (UGG) by the translation machinery. This edit allows translation to occur all the way to the end of the reporter mRNA and results in the expression of Renilla luciferase. Conversely, in the absence of ADAR1 activity, translation terminates at the stop codon and Renilla is not expressed. Reporter activity is read out as the Renilla Luciferase/Firefly luciferase ratio whereby inhibition of ADAR activity, and thus the UAG (stop) to UGG (tryptophan) conversion rate, will result in a dose-dependent decrease in the Renilla luciferase/Firefly luciferase ratio.

Luciferase Assay Kit
G287 500 Reactions
EUR 145

Luciferase Assay Reagent
AR4012 1 kit, 1000 tests (96-well plate format)
EUR 850

Firefly Luciferase Assay Kit
MBS434279-1Plate 1Plate
EUR 210

Firefly Luciferase Assay Kit
MBS434279-5x1Plate 5x1Plate
EUR 960

LucyQ Firefly Luciferase Assay Kit
L0010-010 100 Assays
EUR 172

LucyQ Firefly Luciferase Assay Kit
L0010-100 1000 Assays
EUR 440

LucyQ Gaussia Luciferase Assay Kit
L0011-010 100 Assays
EUR 185

LucyQ Gaussia Luciferase Assay Kit
L0011-100 1000 Assays
EUR 700